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Annals of Botany 68: 243-245, 1991
© 1991 Annals of Botany Company

RESEARCH-ARTICLE

In Vitro Micropropagation of Delphinium malabaricum (Huth) Munz.—a Rare Species*

D C AGRAWAL, S S PAWAR, G. C MORWAL and A F MASCARENHAS

Division of Biochemical Sciences, National Chemical Laboratory Pune 411008, India

Accepted: 29 April 1991   

A micropropagation technique was developed for Delphinium malabaricum using nodes from inflorescence stalks Maximum shoot proliferation was obtained on Murashige and Skoog's medium supplemented with 2-1P (10 mg l–1) and inositol (100 mg l–1) From the sixth passage onwards, shoots could be multiplied by omission of inositol and reduction of 2-1P (0.5 mg l–1) concentration Best rooting response was obtained with a 24-h pulse treatment of shoots with 0.5 mg I–1 IBA in the dark, transfer of treated shoots to hormone-free half-strength MS medium and incubation under 24-h light. Regenerated plants were established successfully in the field Cytological examination of root tips of in vitro and control plants showed identical chromosome number (2n = 16)

Delphinium malabaricum (Huth) Munz, micropropagation, tissue culture, rare plant


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