Skip Navigation



Journal Molluscan Studies Advance Access published online on November 6, 2007
Journal of Molluscan Studies, doi:10.1093/mollus/eym036
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
73/4/393    most recent
eym036v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Huang, X.
Right arrow Articles by Bao, Z.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Huang, X.
Right arrow Articles by Bao, Z.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2007. Published by Oxford University Press on behalf of The Malacological Society of London, all rights reserved

MAPPING OF RIBOSOMAL DNA AND (TTAGGG)n TELOMERIC SEQUENCE BY FISH IN THE BIVALVE PATINOPECTEN YESSOENSIS (JAY, 1857)

Xiaoting Huang, Xiaoli Hu, Jingjie Hu, Lingling Zhang, Shi Wang, Wei Lu and Zhenmin Bao

Laboratory of Marine Genetics and Breeding (MGB), Division of Life Science and Technology, Ocean University of China, Qingdao 266003, China

Correspondence: Z. Bao; e-mail: zmbao{at}ouc.edu.cn


  Abstract

The chromosome set of Patinopecten yessoensis (Jay, 1857) was characterized using Giemsa staining, DAPI staining and fluorescence in situ hybridization (FISH) with three repetitive DNA probes [18S–28S rDNA, 5S rDNA and telomeric (TTAGGG)n]. DAPI staining showed that AT-rich regions were located on the centromere of almost all chromosomes and interstitial banding was not observed. FISH showed that 18S–28S rDNA spread over the short arms of two subtelocentric chromosome pairs and 5S rDNA was located on the long arm of one subtelocentric chromosome pair. Sequential FISH demonstrated that 18S–28S and 5S rDNA were located on different chromosomes. FISH also showed that the vertebrate telomeric sequence (TTAGGG)n was located on both ends of each chromosome and no interstitial signals were detected. Sequential 18S–28S rDNA and (TTAGGG)n FISH indicated that repeated units of the two multicopy families were closely associated on the same chromosome pair.

(Received 4 January 2007; accepted 1 September 2007)


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.